Product Name

Neuraminidase from Vibrio cholerae

General deion

Neuraminidase enzymes are glycoside hydrolase enzymes that catalyze hydrolysis of terminal sialic acid residues. The most well-known are the viral nearamidases, which promote influenza virus release.

Synonym

Acyl-neuraminyl Hydrolase, Receptor-destroying enzyme, Sialidase

Assay

Type III, 0.2 μm filtered, 1-5 units/mg protein (Lowry, using NAN-lactose)

CAS Number

9001-67-6

MDL number

MFCD00131711

Suitability

BioReagent

l Physical and Chemical Information

Appearance

buffered aqueous solution

Physical form

Aqueous solution, pH 5.5, containing 0.15 M NaCl and 4 mM CaCl².

l Biological Information

Biochem/Physiol Actions

Vibrio cholerae neuraminidase has been shown to promote cholera toxin infection by binding the toxin and aiding in its uptake by cells.

Application

Neurminidase is used as a cell-surface probe for glycoconjugate distribution and in substrate specificity studies.

Neuraminidase from Vibrio cholerae has been used in a study to assess its role in the binding and uptake of cholera toxin by susceptible cells.It has also been used in a study to investigate the preparation of a sodium substrate and its use in a fluorometric assay of neuraminidase.

Preparation Note

Chromatographically purified.

Unit Definition

One unit will liberate 1.0 μmole of N-acetylneuraminic acid per min at pH 5.0 at 37 °C using NAN-lactose or bovine submaxillary mucin, unless otherwise specified. Prices based on units using NAN-lactose as substrate.

l Storage

Storage temp.

2-8°C

l Precautions and Disclaimer

This product is for R&D use only, not for drug, household, or other uses.

l References

1. http://www.drugbank.ca

2. https://ncit.nci.nih.gov

3. https://www.ncbi.nlm.nih.gov