Product Name

Neuraminidase from Vibrio cholerae

General deion

Calorimetry studies show that the two lectin-like domains flanking the central catalytic domain of Neuraminidase serve as the recognition and binding sites for sialic acid-containing substrates.

Synonym

Acyl-neuraminyl Hydrolase, Receptor-destroying enzyme, Sialidase

Assay

Type II, 8-24 units/mg protein (Lowry, using NAN-lactose)

CAS Number

9001-67-6

MDL number

MFCD00131711

Suitability

BioReagent

l Physical and Chemical Information

Appearance

buffered aqueous solution

Physical form

Solution in 50 mM sodium acetate, pH 5.5, containing 0.15 M sodium chloride and 4 mM calcium chloride, 0.2 μm filtered.

l Biological Information

Application

Neurminidase is used as a cell-surface probe for glycoconjugate distribution and in substrate specificity studies. Neuraminidase from Vibrio cholera has been used in a study to describe a five-step purification method. It has also been used in a study to investigate modification of leukemia L1210 tumor cells.

Preparation Note

A further purification by affinity chromatography of our Type III.

Unit Definition

One unit will liberate 1.0 μmole of N-acetylneuraminic acid per min at pH 5.0 at 37 °C using NAN-lactose or bovine submaxillary mucin, unless otherwise specified. Prices based on units using NAN-lactose as substrate.

l Storage

Storage temp.

2-8°C

l Precautions and Disclaimer

This product is for R&D use only, not for drug, household, or other uses.

l References

1. http://www.drugbank.ca

2. https://ncit.nci.nih.gov

3. https://www.ncbi.nlm.nih.gov