Product Name

Neuraminidase Agarose from Clostridium perfringens (C. welchii)

General deion

Neuraminidase enzymes are glycoside hydrolase enzymes that catalyze hydrolysis of terminal sialic acid residues. The most well-known are the viral nearamidases, which promote influenza virus release.

Synonym

Acylneuraminyl hydrolase, Receptor-destroying enzyme, Sialidase

Assay

Type VI-A; 0.6-1.8 units per mL gel; 20-60 units per g agarose

MDL number

MFCD00131711

Suitability

BioReagent

l Physical and Chemical Information

Appearance

ammonium sulfate suspension

Physical form

Suspension in 2.0 M (NH⁴)²SO⁴ solution, pH 7.0.

l Biological Information

Application

Agarose-ed neuraminidase from Clostridium perfringens can be used for the detection of total and desialylated sex hormone-binding globulin in human serum samples. Neuraminidase from Clostridium perfringens has been used in a study to assess a genetic polymorphism of human serum glycoprotein (inter-α-trypsin-inhibitor). It has also been used in a study to investigate the inhibition of neuraminidase by chemically sulphated glycopeptides.

Preparation Note

Prepared from Neuraminidase, Type VI.

Unit Definition

One unit will liberate 1.0 μmole of N-acetylneuraminic acid per min at pH 5.0 at 37 °C using NAN-lactose or bovine submaxillary mucin, unless otherwise specified. Prices based on units using NAN-lactose as substrate.

l Storage

Storage temp.

2-8°C

l Precautions and Disclaimer

This product is for R&D use only, not for drug, household, or other uses.

l References

1. http://www.drugbank.ca

2. https://ncit.nci.nih.gov

3. https://www.ncbi.nlm.nih.gov