Product Name

α(2→3,6,8,9) Neuraminidase from Arthrobacter ureafaciens

Synonym

Acyl-neuraminyl Hydrolase, Neuraminidase from Arthrobacter ureafaciens, Sialidase

Assay

Proteomics Grade, suitable for MALDI-TOF MS; ≥25 U/vial

CAS Number

9001-67-6

MDL number

MFCD00131711

Suitability

BioReagent

l Physical and Chemical Information

Appearance

Lyophilized Powder

l Biological Information

Application

Neuraminidase is an important deglycosylation enzyme capable of cleaving all non-reducing unbranched N-acetylneuraminic and N-glycolylneuraminic acid residues by hydrolysis of α(2→6), α(2→3), α(2→8), and α(2→9) ages (affinity in the order given). Branched sialic acids may also be cleaved with the use of high concentrations of enzyme and prolonged incubations. Desialylated glycoproteins may then be further characterized by treatment with various exoglycosidases resulting in partial or complete O-deglycosylation. SDS-PAGE and MALDI-TOF MS are typically utilized in purification, structural analysis, and sequencing process. These techniques also remove heterogeneity and charge from the glycoprotein.

Unit Definition

One unit will release 1 nmole of 4-methylumbelliferone from 2-(4-methylumbelliferyl) α-D-N-acetylneuraminic acid per minute at pH 5.5 at 37° C.

l Storage

Storage temp.

2-8°C

l Precautions and Disclaimer

This product is for R&D use only, not for drug, household, or other uses.

l References

1. http://www.drugbank.ca

2. https://ncit.nci.nih.gov

3. https://www.ncbi.nlm.nih.gov