Product Name

Lysozyme from chicken egg white

Synonym

Mucopeptide N-acetylmuramoylhydrolase, Muramidase

Assay

Protein:≥90%; ≥40,000 units/mg protein; aseptically filled

CAS Number

12650-88-3

Molecular Weight

single-chain mol wt 14.3 kDa

MDL number

MFCD00131557

Suitability

BioReagent

l Physical and Chemical Information

Appearance

White powder

Solubility(25°C)

10 mg/mL (H²O); Colorless; Clear to Slightly Hazy

l Biological Information

Biochem/Physiol Actions

Lysozyme hydrolyzes β(1→4) ages between N-acetylmuramic acid and N-acetyl-D-glucosamine residues in peptidoglycan and between N-acetyl-D-glucosamine residues in chitodextrin. Gram-positive cells are quite susceptible to this hydrolysis as their cell walls have a high proportion of peptidoglycan. Gram-negative bacteria are less susceptible due to the presence of an outer membrane and a lower proportion of peptidoglycan. However, these cells may be hydrolyzed in the presence of EDTA that chelates metal ions in the outer bacterial membrane.

The enzyme is active over a broad pH range (6.0 to 9.0). At pH 6.2, maximal activity is observed over a wider range of ionic strengths (0.02 to 0.100 M) than at pH 9.2 (0.01 to 0.06 M).

Application

Enzyme breaks down the cell walls of bacteria; used to prepare spheroplasts.

Preparation Note

For E. coli cell lysis, use a freshly prepared lysozyme solution (10 mg/ml) in 10 mM Tris-HCl, pH 8.0.

The product is also soluble in water (10 mg/ml) yielding a clear to slightly hazy colorless solution. Aqueous solutions should retain activity for at least one month when stored between 2–8 °C.

Unit Definition

One unit will produce a ΔA⁴⁵⁰ of 0.001 per min at pH 6.24 at 25 °C, using a suspension of Micrococcus lysodeikticus as substrate, in a 2.6 mL reaction mixture (1 cm light path).

l Storage

Storage temp.

-20°C

l Precautions and Disclaimer

This product is for R&D use only, not for drug, household, or other uses.

l References

1. http://www.drugbank.ca

2. https://ncit.nci.nih.gov

3. https://www.ncbi.nlm.nih.gov