Product Name

Lysozyme from chicken egg white

Synonym

Mucopeptide N-acetylmuramoylhydrolase, Muramidase

Assay

protein ≥90 %, ≥40,000 units/mg protein

CAS Number

12650-88-3

Molecular Weight

single-chain mol wt 14.3 kDa

MDL number

MFCD00131557

Suitability

BioReagent

l Physical and Chemical Information

Appearance

White to Off-White Powder lyophilized powder

Solubility(25°C)

10 mg/mL (H2O), Colorless, Clear to Slightly Hazy.

l Biological Information

Biochem/Physiol Actions

Lysozyme hydrolyzes β(1→4) ages between N-acetylmuramic acid and N-acetyl-D-glucosamine residues in peptidoglycan and between N-acetyl-D-glucosamine residues in chitodextrin. Gram-positive cells are quite susceptible to this hydrolysis as their cell walls have a high proportion of peptidoglycan. Gram-negative bacteria are less susceptible due to the presence of an outer membrane and a lower proportion of peptidoglycan. However, these cells may be hydrolyzed in the presence of EDTA that chelates metal ions in the outer bacterial membrane.

The enzyme is active over a broad pH range (6.0 to 9.0). At pH 6.2, maximal activity is observed over a wider range of ionic strengths (0.02 to 0.100 M) than at pH 9.2 (0.01 to 0.06 M).

Application

Enzyme breaks down the cell walls of bacteria; used to prepare spheroplasts.

Lysozyme from chicken egg white has been used for the extraction of genomic DNA from bacterial cells. It has been used as an external standard for MALDI-TOF (matrix assisted laser desorption ionization-time of flight) mass analysis.

Preparation Note

For E. coli cell lysis, use a freshly prepared lysozyme solution (10 mg/ml) in 10 mM Tris-HCl, pH 8.0.1

Unit Definition

One unit will produce a ΔA⁴⁵⁰ of 0.001 per min at pH 6.24 at 25 °C, using a suspension of Micrococcus lysodeikticus as substrate, in a 2.6 mL reaction mixture (1 cm light path).

l Storage

Storage temp.

-20°C

l Precautions and Disclaimer

This product is for R&D use only, not for drug, household, or other uses.

l References

1. http://www.drugbank.ca

2. https://ncit.nci.nih.gov

3. https://www.ncbi.nlm.nih.gov