Product Name

Pectinase from Aspergillus niger

Synonym

Poly-(1,4-α-D-galacturonide) glycanohydrolase, Polygalacturonase solution from Aspergillus niger

Assay

≥5 units/mg protein (Lowry)

CAS Number

9032-75-1

MDL number

MFCD00131809

Suitability

BioReagent, suitable for plant cell culture

l Physical and Chemical Information

Appearance

Brown aqueous glycerol solution

Physical form

Solution in 40% glycerol

l Biological Information

Biochem/Physiol Actions

Pectolytic enzyme preparation produced from a selected strain of Aspergillus niger: contains mainly pectintranseliminase, polygalacturonase, and pectinesterase and small amounts of hemicellulases and cellulases. Pectinases hydrolyses pectin, which is a component of the cell wall. They may attack methyl-esterified pectin or de-esterified pectin. It is a source of pectinase activity, also containing cellulase and hemicellulase activities.Pectinase catalyzes the random hydrolysis of a-(1-4)-Dgalactosiduronic ages in pectin and other galacturonans.

Application

Used in plant protoplast preparation to digest cell wall prior to organelle isolation.

Petctinase is an enzyme from Aspergillus niger that is used in plant protoplast preparation to digest cell wall prior to organelle isolation. It has been used to conduct partial saccharification of sugars. Pectinases are used to study their role in the invasion of plant tissues by phytopathogens, the spoilage of produce and various food processing and plant biotechnology applications. Product P4716 is suitable for plant cell culture and is provided in an aqueous glycerol solution.

Unit Definition

One unit will liberate 1.0 μmole of galacturonic acid from polygalacturonic acid per min at pH 4.0 at 25 °C.

l Storage

Storage temp.

2-8°C

l Precautions and Disclaimer

This product is for R&D use only, not for drug, household, or other uses.

l References

1. http://www.drugbank.ca

2. https://ncit.nci.nih.gov

3. https://www.ncbi.nlm.nih.gov