Product Name

Pectinase from Rhizopus sp.

Synonym

Macerozyme R-10, Poly-(1,4-α-D-galacturonide) glycanohydrolase, Polygalacturonase

Assay

400-800 units/g solid

CAS Number

9032-75-1

MDL number

MFCD00131809

Suitability

BioReagent

l Physical and Chemical Information

Appearance

powder

l Biological Information

Biochem/Physiol Actions

Pectinases hydrolyses pectin, which is a component of the cell wall. They may attack methyl-esterified pectin or de-esterified pectin. It is a source of pectinase activity, also containing cellulase and hemicellulase activities.

Application

Used in plant protoplast preparation to digest cell wall prior to organelle isolation.

Petctinase is an enzyme from Rhizopus sp. that is used in plant protoplast preparation to digest cell wall prior to organelle isolation. It has been used to digest the cell wall of Arabidopsis root cells to study peroxin 16 in peroxisomes and endoplasmic reticulum. Pectinases are used to study their role in the invasion of plant tissues by phytopathogens, the spoilage of produce and various food processing and plant biotechnology applications.

Unit Definition

One unit will liberate 1.0 μmole of galacturonic acid from polygalacturonic acid per min at pH 4.0 at 25 °C.

l Storage

Storage temp.

-20°C

l Precautions and Disclaimer

This product is for R&D use only, not for drug, household, or other uses.

l References

1. http://www.drugbank.ca

2. https://ncit.nci.nih.gov

3. https://www.ncbi.nlm.nih.gov