Product Name

Thioglucosidase from Sinapis alba (white mustard) seed

General deion

Thioglucosidase research has focused mainly on the cruciferous crops due to their economic importance and cancer preventive benefits.

Synonym

Glucosinolase, Myrosinase, Thioglucoside glucohydrolase

Assay

≥100 units/g solid

CAS Number

9025-38-1

MDL number

MFCD00132422

Suitability

BioReagent

l Physical and Chemical Information

Appearance

solid

l Biological Information

Biochem/Physiol Actions

Myrosinases are present in many bacteria, fungi, and edible plants, including those of the Brassicaceae (Cruciferae) family. The enzyme hydrolyzes the S-glucosidic bond of a glucosinolate substrate to form an unstable aglycone that rearranges with the loss of sulfate primarily to the isothiocyanate, though thiocyanates and nitriles are also formed. Many of the isothiocyanate products of aliphatic and aromatic glucosinolates have cancer chemopreventive properties.

Application

Thioglucosidase has been used in a study to assess Brassica species screening for glucosinolate content.  Thioglucosidase has also been used in a study to investigate a negative regulatory role for auxin in sulphate deficiency response in Arabidopsis thaliana.

Unit Definition

One unit will produce 1.0 μmole glucose per min from sinigrin at pH 6.0 at 25 °C.

l Storage

Storage temp.

-20°C

l Precautions and Disclaimer

This product is for R&D use only, not for drug, household, or other uses.

l References

1. http://www.drugbank.ca

2. https://ncit.nci.nih.gov

3. https://www.ncbi.nlm.nih.gov