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Chitinase from Streptomyces griseus

Product #: S0451
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Details

l General Information

Product Name

Chitinase from Streptomyces griseus

Synonym

Chitodextrinase, Poly(1,4-β-[2-acetamido-2-deoxy-D-glucoside]) glycanohydrolase

Assay

≥200 units/g solid

CAS Number

9001-06-3

MDL number

MFCD00130771

Suitability

BioReagent

l Physical and Chemical Information

Physical form

Tan to Brown lyophilized powder (essentially salt free)

Solubility(25°C)

H2O: soluble 0.90-1.10 mg/mL

l Biological Information

Biochem/Physiol Actions

Chitinase is an extracellular enzyme complex that degrades chitin and has a molecular mass of approximately 30 kDa. Chitin is degraded to N-acetyl-D-glucosamine in 2 enzymatic reactions. Firstly, chitobiose units are removed from chitin by chitodextrinase-chitinase. The second reaction involves N-acetyl-glucosaminidase-chitobiase, which cleaves the disaccharide to its monomer subunits (that comprise of N-acetyl-D-glucosamine). The optimum reaction temperature is 37 °C.

Application

Chitinase from Streptomyces griseus has been used to study the effect of the allosamidin on the regulatory system for chitinase production. It has also been used to study the enrichment of chitinolytic microorganisms. This enrichment was achieved by the isolation and characterization of a chitinase with antifungal activity against phytopathogenic fungi. Chitinase from Streptomyces griseus has been used as a standard for chitinase assays using the enzyme derived from Absidia glauca and Petunia hybrida extracts.

Unit Definition

One unit will liberate 1.0 mg of N-acetyl-D-glucosamine from chitin per hour at pH 6.0 at 25 °C in a 2 hour assay.

One new 1 hour unit = approx. 50 old 48 hour units.

l Storage

Storage temp.

-20°C

l Precautions and Disclaimer

This product is for R&D use only, not for drug, household, or other uses.

l References

1. http://www.drugbank.ca

2. https://ncit.nci.nih.gov

3. https://www.ncbi.nlm.nih.gov

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