Product Name

Chitosanase from Streptomyces griseus

Synonym

Chitosan N-acetylglucosaminohydrolase

Assay

>50 units/mg protein (Bradford); chitinase <1.0 unit/mg protein

CAS Number

51570-20-8

MDL number

MFCD00211797

Suitability

BioReagent

l Physical and Chemical Information

Appearance

White lyophilized powder

Physical form

Lyophilized powder containing potassium phosphate buffer salts

Solubility(25°C)

H²O: soluble, clear to slightly hazy, colorless (10U/0.3mL)

l Biological Information

Biochem/Physiol Actions

Chitosanase catalyzes the endohydrolysis of β(1,4) ages between N-acetyl-D-glucosamine and D-glucosamine residues in partially deacetylated chitosan. Chitosanase from Streptomyces griseus is capable of hydrolyzing both chitosan and carboxymethyl cellulose. It is used for the lysis of cell walls of fungi belonging to the group Mucorales. It is found in several types of microorganisms.

Application

Chitosanase from Streptomyces griseus has been used in a study to assess the effect of chitin sources on production of chitinase and chitosanase. Chitosanase from Streptomyces griseus has also been used in a study to investigate the effective production of chitinase and chitosanase by Streptomyces griseus HUT 6037 using colloidal chitin and various degrees of deacetylation of chitosan.

The enzyme from Sigma has been used for the hydrolysis of the crude cell wall preparation of oomycete Aphanomyces euteiches, a major parasite of legume plants. It has also been used for the enzymatic hydrolysis of the fully de-N-acetylated chitosan to get chitosan oligomer mixtures during the preparation of biocompatible chitosan-alginate gel.

Preparation Note

For long term solution storage, reconstitute vial with 50% glycerol solution. Dilute to working concentrations with a 0.1% BSA solution.

Unit Definition

One unit will release 1 μmole of glucosamine from chitosan per min at pH 5.0 at 37 °C as measured in the fluorimetric assay of Osswald, et al. (1992).

l Storage

Storage temp.

-20°C

l Precautions and Disclaimer

This product is for R&D use only, not for drug, household, or other uses.

l References

1. http://www.drugbank.ca

2. https://ncit.nci.nih.gov

3. https://www.ncbi.nlm.nih.gov