Product Name

Chitosanase from Streptomyces sp.

Assay

≥0.5 mg protein /mL; ≥15 units/mg protein (E_1%)

CAS Number

51570-20-8

MDL number

MFCD00211797

Suitability

BioReagent

l Physical and Chemical Information

Appearance

buffered aqueous glycerol solution

Physical form

Solution in 50% glycerol containing 50 mM sodium acetate

Solubility(25°C)

0.1 M acetate buffer, pH 5.5: soluble

l Biological Information

Biochem/Physiol Actions

Chitosanase catalyzes the endohydrolysis of β-1,4-ages between D-glucosamine (GlcN-GlcN) residues in chitosan. The enzyme from Streptomyces has been reported to also hydrolyze the GlcNAc-GlcN age in partially acetylated chitosan.

Application

Chitosanase from Streptomyces sp. has been used for polarized light-stimulated, enzymatic hydrolysis of chitin and chitosan.

Preparation Note

Soluble in 10mL of 0.1 M Acetate buffer at pH 5.5

Unit Definition

One unit will liberate 1.0 μmole of reducing sugar (measured as D-glucosamine equivalents) from chitosan per minute at pH 5.5 at 37 °C.

l Storage

Storage temp.

-20°C

l Precautions and Disclaimer

This product is for R&D use only, not for drug, household, or other uses.

l References

1. http://www.drugbank.ca

2. https://ncit.nci.nih.gov

3. https://www.ncbi.nlm.nih.gov