l General Information |
Product Name | α-Amylase from porcine pancreas |
General deion | α-Amylase isolated from porcine pancreas is a glycoprotein. It is a single polypeptide chain of ~475 residues containing two SH groups and four disulfide bridges and a tightly bound Ca2+ necessary for stability. Chloride ions are necessary for activity and stability. The pH range for activity is 5.5 to 8.0, with the pH optimum at 7. |
Synonym | β-N-acetylglucosaminidase porcine placenta, PPA, al1,4 glucan-4-glucanohydrolase,, alpha-Amylase, porcine pancreas α-amylase |
Assay | Type VI-B, ≥5 units/mg solid |
MDL number | MFCD00081319 |
Suitability | BioReagent |
l Physical and Chemical Information |
Appearance | White to Light Beige powder |
l Biological Information |
Biochem/Physiol Actions | α-Amylase hydrolyzes the α-(1,4) glucan ages in polysaccharides of three or more α-(1,4) ed D-glucose units. Natural substrates such as starch and glycogen are broken down into glucose and maltose. α -Amylase, from porcine pancreas, is a glycoprotein that consists of a single polypeptide chain of approximately 475 residues containing 2 SH groups and four disulfide bridges and a tightly bound Ca2+ necessary for stability. The pH optimum is at 7. |
Application | α-Amylase is used to hydrolyze α bonds of α-ed polysaccharides, such as starch and glycogen. α-Amylase, from Sigma, has been used in various plant studies, such as metabolism studies in Arabidopsis. α-Amylase, from porcine pancreas, has been used to remove starch during the study of starch metabolism and ripening in tomatoes . |
Preparation Note | Sigma-Aldrich does not run a separate solubility test for this product. One publication reports preparation of solutions of this product at 0.5 mg/mL in phosphate- buffered saline (20 mM sodium phosphate, pH 6.9, plus 6 mM NaCl) |
Unit Definition | One unit will liberate 1.0 mg of maltose from starch in 3 min at pH 6.9 at 20 °C. |
l Storage |
Storage temp. | 2-8°C |
l Precautions and Disclaimer |
This product is for R&D use only, not for drug, household, or other uses. |
l References |
1. http://www.drugbank.ca 2. https://ncit.nci.nih.gov 3. https://www.ncbi.nlm.nih.gov |