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Home > Products > Life sciences > Enzymes and proteins > α-Amylase from porcine pancreas

α-Amylase from porcine pancreas

Product #: S0431
Image SKU-Pack Size Stock Price($) Quantity
  • S0431-500KU

    In-stock

    122

    - +

    S0431-1MU

    In-stock

    178.2

    - +

    S0431-2.5MU

    In-stock

    340.6

    - +

    S0431-5MU

    In-stock

    577.2

    - +

    S0431-10MU

    In-stock

    894.4

    - +

Documents

Details

l General Information

Product Name

α-Amylase from porcine pancreas

General deion

α-Amylase isolated from porcine pancreas is a glycoprotein. It is a single polypeptide chain of ~475 residues containing two SH groups and four disulfide bridges and a tightly bound Ca2+ necessary for stability. Chloride ions are necessary for activity and stability. The pH range for activity is 5.5 to 8.0, with the pH optimum at 7.

Synonym

β-N-acetylglucosaminidase porcine placenta, PPA, al1,4 glucan-4-glucanohydrolase,, alpha-Amylase, porcine pancreas α-amylase

Assay

Type VI-B, ≥5 units/mg solid

MDL number

MFCD00081319  

Suitability

BioReagent

l Physical and Chemical Information

Appearance

White to Light Beige powder

l Biological Information

Biochem/Physiol Actions

α-Amylase hydrolyzes the α-(1,4) glucan ages in polysaccharides of three or more α-(1,4) ed D-glucose units. Natural substrates such as starch and glycogen are broken down into glucose and maltose. α -Amylase, from porcine pancreas, is a glycoprotein that consists of a single polypeptide chain of approximately 475 residues containing 2 SH groups and four disulfide bridges and a tightly bound Ca2+ necessary for stability. The pH optimum is at 7.

Application

α-Amylase is used to hydrolyze α bonds of α-ed polysaccharides, such as starch and glycogen. α-Amylase, from Sigma, has been used in various plant studies, such as metabolism studies in Arabidopsis. α-Amylase, from porcine pancreas, has been used to remove starch during the study of starch metabolism and ripening in tomatoes .

Preparation Note

Sigma-Aldrich does not run a separate solubility test for this product. One publication reports preparation of solutions of this product at 0.5 mg/mL in phosphate- buffered saline (20 mM sodium phosphate, pH 6.9, plus 6 mM NaCl)

Unit Definition

One unit will liberate 1.0 mg of maltose from starch in 3 min at pH 6.9 at 20 °C.

l Storage

Storage temp.

2-8°C

l Precautions and Disclaimer

This product is for R&D use only, not for drug, household, or other uses.

l References

1. http://www.drugbank.ca

2. https://ncit.nci.nih.gov

3. https://www.ncbi.nlm.nih.gov

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