Product Name

Phospholipase C, Phosphatidylinositol-specific from Bacillus cereus

General deion

Phosphatidylinositol-specific phospholipase C (PI-PLC) from Bacillus cereus is a 298 residual protein. It belongs to the class of ubiquitous enzymes. PI-PLC is characterized with a single globular domain and it folds into eight (βα) barrel sheets, out of which only six βα units are found in barrel, lacking helices between strands IV and V and V and VI.

Synonym

1-Phosphatidyl-D-myo-inositol inositol phosphohydrolase, cyclic-phosphate forming, PI-PLC

Assay

Protein: ≤1%;

≥1,000 units/mg protein (Lowry);

Phopholipase C (lecithinase) ≤1 units/mg protein ;

Sphingomyelinase ≤40 units/mg protein

CAS Number

37288-19-0

MDL number

MFCD00131896

Suitability

BioReagent

l Physical and Chemical Information

Appearance

White to Off White lyophilized powder

Physical form

Contains phosphate buffer salts, EDTA and stabilizer

l Biological Information

Biochem/Physiol Actions

Used for the release of GPI anchored proteins from the membrane.

Application

Phospholipase C, Phosphatidylinositol-specific from Bacillus cereus has been used to cleave GPI-anchored protein from green fluorescent protein- glycosylphosphatidylinositol (GFP-GPI) from the cell membrane.It has also been used in invasion assay to test the involvement of PLAP in T. cruzi invasion to human epithelial type 2 (HEp-2) cells.

Preparation Note

The enzyme can be solubilized in 10 mM Tris-HCl, pH 7.4, containing 144 mM NaCl and 0.05% BSA.

Unit Definition

One unit will liberate one unit of acetylcholinesterase per minute from a membrane-bound crude preparation at pH 7.4 at 30 °C (10 minute incubation).

l Storage

Storage temp.

-20°C

l Precautions and Disclaimer

This product is for R&D use only, not for drug, household, or other uses.

l References

1. http://www.drugbank.ca

2. https://ncit.nci.nih.gov

3. https://www.ncbi.nlm.nih.gov