Product Name

Sulfatase from abalone entrails

Synonym

Aryl-sulfatase, Aryl-sulfate sulfohydrolase, Phenolsulfatase

Assay

20-40 units/mg solid; Type VIII; β-Glucuronidase ≤10.0 units/mg solid

CAS Number

9016-17-5

MDL number

MFCD00132399

Suitability

BioReagent

l Physical and Chemical Information

Appearance

lyophilized powder

l Biological Information

Application

Sulfatase from abalone entrails has been used in a study to determine that human p-selectin glycoprotein ligand-1 interacts with the skin-associated chemokine CCL27 via sulfated tyrosines at the PSGL-1 amino terminus.  Sulfatase from abalone entrails has also been used in a study to investigate HPLC of sulfate and glutathione conjugates from hamster embryo fibroblasts.

Preparation Note

Stock solutions of this product may be prepared in 0.2% NaCl aqueous solution. One publication reports preparation of 500 units/mL aqueous stock solutions of this product in 100 mM sodium acetate, pH 7.0, and storage of this stock solution in aliquots at –80 °C.

Unit Definition

One unit will hydrolyze 1.0 μmole of p-nitrocatechol sulfate per hr at pH 5.0 at 37 °C (30 min assay).

l Storage

Storage temp.

-20°C

l Precautions and Disclaimer

This product is for R&D use only, not for drug, household, or other uses.

l References

1. http://www.drugbank.ca

2. https://ncit.nci.nih.gov

3. https://www.ncbi.nlm.nih.gov