Product Name

Sulfatase from Helix pomatia

Synonym

Aryl-sulfatase, Aryl-sulfate sulfohydrolase, Phenolsulfatase

Assay

Sulfatase≥10,000 units/g solid; Type H-1; ≥300 unit/mg solid β-glucuronidase (at pH 5.0)

CAS Number

9016-17-5

MDL number

MFCD00132399

Suitability

BioReagent

l Physical and Chemical Information

Appearance

White to Light Yellow to Light Brown powder

l Biological Information

Biochem/Physiol Actions

Sulfatase from Helix pomatia facilitates the synthesis of thiohydroximates. It causes the hydrolysis of β-naphthyl sulfate much faster than a-naphthyl sulfate and possesses regiospecificity in the hydrolysis of ortho and para substituted phenyl sulfates.

Preparation Note

One publication reports preparation of solutions of this sulfatase product at 100 units/mL in 200 mM sodium acetate buffer with 20 mM saccharic acid 1,4-lactone.

Unit Definition

One unit will hydrolyze 1.0 μmole of p-nitrocatechol sulfate per hour at pH 5.0 at 37 °C (30 min assay).

l Storage

Storage temp.

-20°C

l Precautions and Disclaimer

This product is for R&D use only, not for drug, household, or other uses.

l References

1. http://www.drugbank.ca

2. https://ncit.nci.nih.gov

3. https://www.ncbi.nlm.nih.gov