Phospholipase D from cabbage
Product #:
S0397
Image
SKU-Pack Size
Stock
Price($)
Quantity
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S0397-500UN
In-stock
405.6
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S0397-2.5KU
In-stock
1580.8
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Details
l General Information |
Product Name | Phospholipase D from cabbage |
Synonym | Lecithinase D, Phosphatidylcholine phosphatidohydrolase, Phospholipase D from white cabbage |
Assay | ≥100 units/mg solid; Type IV |
CAS Number | 9001-87-0 | MDL number | MFCD00131897 |
Suitability | BioReagent |
l Physical and Chemical Information |
Appearance | lyophilized powder |
l Biological Information |
Biochem/Physiol Actions | Hydrolyzes the phosphate bonds of phospholipids and sphingomyelin to give the corresponding phosphatidic acid. The amino acid sequence of cabbage PLD has 83% and 80% identity with Arabidopsis PLDα and castor bean PLD. Hydrolyzes the phosphate bonds of phospholipids and sphingomyelin to give the corresponding phosphatidic acid. |
Application | Phospholipase D (PLD) is used to hydrolyze the phosphate bonds of phospholipids and sphingomyelin to give the corresponding phosphatidic acid. It has also been used to study metabolic labeling and direct imaging of choline phospholipids in vivo by measuring propargyl-Cho incorporation. Furthermore, PLD is used in purification and kinetic studies. The enzyme has been used for the preparation of Bodipy-phosphatidylcholine during the preparation of fluorescently labelled lipids. |
Unit Definition | One unit will liberate 1.0 μmol of choline from L-α-phosphatidylcholine (egg yolk) per hr at pH 5.6 at 30 °C. |
l Storage |
Storage temp. | -20°C |
l Precautions and Disclaimer |
This product is for R&D use only, not for drug, household, or other uses. |
l References |
1. http://www.drugbank.ca 2. https://ncit.nci.nih.gov 3. https://www.ncbi.nlm.nih.gov |