Product Name

Phospholipase D from Arachis hypogaea (peanut)

General deion

Phospholipase D is a phospholipid hydrolyzing enzyme and an important component of receptor-mediated signal transduction responses and regulated secretion.

Synonym

Lecithinase D, Phosphatidylcholine phosphatidohydrolase

Assay

Protein: 20% - 40%;≥60 units/mg protein; Type II

CAS Number

9001-87-0

MDL number

MFCD00131897

Suitability

BioReagent

l Physical and Chemical Information

Appearance

White to Off-white to Yellow Cast lyophilized powder

Physical form

Partially purified, lyophilized powder containing buffer salts

l Biological Information

Biochem/Physiol Actions

Hydrolyzes the phosphate bonds of phospholipids and sphingomyelin to give the corresponding phosphatidic acid.

Phospholipase D is involved in conferring drought susceptibility in peanuts, which increases the risk of aflatoxin contamination.

Application

Research has shown ADP-ribosylation factor regulation of phospholipase D is important in the release of nascent secretory vesicles from the trans-Golgi network. It has also been used in a study to investigate stimulation of Na₊-Ca₂₊ exchange activity in canine cardiac sarcolemmal vesicals.

Unit Definition

One unit will liberate 1.0 μmol of choline from L-α-phosphatidylcholine (egg yolk) per hr at pH 5.6 at 30 °C.

l Storage

Storage temp.

-20 °C

l Precautions and Disclaimer

This product is for R&D use only, not for drug, household, or other uses.

l References

1. http://www.drugbank.ca

2. https://ncit.nci.nih.gov

3. https://www.ncbi.nlm.nih.gov