Product Name

Phospholipase D from Streptomyces chromofuscus

General deion

Phospholipase D (phosphatidylcholine cholinehydrolase, EC 3.1.4.4) is a ubiquitous enzyme with a molecular weight of ~ 50,000Da. It is expressed in a variety of plant tissues, few microorganisms and some mammalian tissues.

Synonym

Lecithinase D, Phosphatidylcholine phosphatidohydrolase

Assay

≥50,000 units/mL

CAS Number

9001-87-0

Molecular Weight

mol wt ~60 kDa

MDL number

MFCD00131897

Suitability

BioReagent

l Physical and Chemical Information

Appearance

buffered aqueous glycerol solution

Physical form

Solution in 100 mM Tris/HCl, pH 8.0, 10% glycerol (v/v), and 0.1% Triton X-100 (w/v).

l Biological Information

Biochem/Physiol Actions

Phospholipase D is active on sphingomyelins and lysophospholipids. Phospholipase D hydrolyzes the phosphate bonds of phospholipids and sphingomyelin to give the corresponding phosphatidic acid.

Phospholipase D (PLD) catalyses the hydrolysis of phosphatidylcholines (PC) to yield phosphatidic acid (PA) and choline.PC-PLD activity is regulated by hormones, cytokines, neurotransmitters and growth factors. Thus, it is considered to have a role in signal transduction in various cell types. Three vital lipid messengers produced by PLD plays a vital role in various crucial cellular processes and these lipids also have an essential role in downstream triglyceride and phospholipid synthetic pathway. PLD is essential for exocytosis in neurons and endocrine cells. In addition, it also plays a key role in phagocytosis.

Application

Phospholipase D from Streptomyces chromofuscus has been used to cleave choline-containing phospholipids (PCs).

Unit Definition

One unit will release 1 μmol of choline from L-α-phosphatidylcholine per hour at pH 8.0 and 37 °C.

l Storage

Storage temp.

−70°C

l Precautions and Disclaimer

This product is for R&D use only, not for drug, household, or other uses.

l References

1. http://www.drugbank.ca

2. https://ncit.nci.nih.gov

3. https://www.ncbi.nlm.nih.gov