Product Name

Ribonuclease H from Escherichia coli

Assay

1,000-4,000 units/mL; recombinant, expressed in E. coli

CAS Number

9050-76-4

MDL number

MFCD00132186

Suitability

BioReagent

l Physical and Chemical Information

Appearance

buffered aqueous glycerol solution

Physical form

Solution in 50% glycerol containing 20 mM Tris-HCl, pH 7.5, 100 mM KCl, 10 mM MgCl², 0.1 mM EDTA, 0.1 mM DTT and 0.05 mg BSA per ml

l Biological Information

Application

Ribonuclease H from Escherichia coli has been used in a study to assess metallobiochemistry of the magnesium ion. Ribonuclease H has also been used in a study to investigate selective inhibitors of HIV-1 reverse tranase associated Rnase H activity.

Unit Definition

One unit hydrolyzes 1.0 nanomole RNA in ₃H-labeled poly(A) • poly(dT) to acid soluble material in 20 min at 37 °C.

l Storage

Storage temp.

-20°C

l Precautions and Disclaimer

This product is for R&D use only, not for drug, household, or other uses.

l References

1. http://www.drugbank.ca

2. https://ncit.nci.nih.gov

3. https://www.ncbi.nlm.nih.gov