Product Name

Phospholipase C from Bacillus cereus

Synonym

Lecithinase C, Lipophosphodiesterase I, PC-PLC, Phosphatidylcholine cholinephosphohydrolase

Assay

Protein: 5% - 15%; ≥200 units/mg protein

CAS Number

9001-86-9

MDL number

MFCD00131895

Suitability

BioReagent

l Physical and Chemical Information

Appearance

White to Light Yellow lyophilized powder

Physical form

Lyophilized powder containing approx. 10% protein. Remainder; trehalose, zinc sulfate, and potassium phosphate

Solubility(25°C)

H²O: soluble 1.0 mg/mL, faintly hazy to hazy

l Biological Information

Biochem/Physiol Actions

Hydrolyzes the phosphate bond on phosphatidylcholine and other glycerophospholipids yielding diacylglycerol; this enzyme will also hydrolyze the phosphate bonds of sphingomyelin, cardiolipin, choline plasmalogen and ceramide phospholipids.

Phospholipase C is induced by thrombin and platelet-activating factor, forming 1,2-diacylglycerol and phosphatidic acid. PLC hydrolyzes the phosphate bond on phosphatidylcholine and other glycerophospholipids yielding diacylglycerol; this enzyme will also hydrolyze the phosphate bonds of sphingomyelin, cardiolipin, choline plasmalogen and ceramide phospholipids.

Application

Phospholipase C (PLC) is used to study adrenoceptor-mediated transmembrane signaling. It is used to degrade inositol-containing phospholipids. Phospholipase C from Bacillus has been used to study how flhF affects the export of PLC and other secretory virulence factors.

The enzyme has been used in the digestion of phosphatidylserine fraction of Schistosoma mansoni adult worms.

Preparation Note

Reconstitution with water (1 mg/ml) will yield a slightly hazy to hazy solution.

Unit Definition

One unit will liberate 1.0 μmole of water soluble organic phosphorus from L-α-phosphatidylcholine per min at pH 7.3 at 37 °C.

l Storage

Storage temp.

-20°C

l Precautions and Disclaimer

This product is for R&D use only, not for drug, household, or other uses.

l References

1. http://www.drugbank.ca

2. https://ncit.nci.nih.gov

3. https://www.ncbi.nlm.nih.gov