Product Name

Phosphodiesterase 5A1 human

Synonym

3′,5′-cyclic-GMP phosphodiesterase, PDE 5A1

Assay

recombinant, expressed in Sf9 cells; ≥0.1 mg protein/ml (Bradford) ; ≥2,000 unit/μg protein

Suitability

BioReagent

l Physical and Chemical Information

Physical form

Supplied as a solution in 20% glycerol containing 40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 0.04% TWEEN-20 and 3 mM DTT

l Biological Information

Biochem/Physiol Actions

PDE5 regulates vascular smooth muscle contraction and is involved in NO-cGMP signaling in platelets to control aggregation. PDE5 may also be involved in the regulation of cGMP signaling in the brain and may modulate pressure-induced cardiac hypertrophy and fibrosis.

Application

Phosphodiesterase (PDE) is an enzyme that is used to breaks phosphodiester bonds. PDE5 is a molecular target for the treatment of erectile dysfunction and pulmonary hypertension. PDE5 is used to find novel PDE5 inhibitors .

Unit Definition

One unit will convert 1.0 picomole of 3′,5′-cGMP to 5′-GMP per minute at pH 7.4 at 37 °C.

l Storage

Storage temp.

−70°C

l Precautions and Disclaimer

This product is for R&D use only, not for drug, household, or other uses.

l References

1. http://www.drugbank.ca

2. https://ncit.nci.nih.gov

3. https://www.ncbi.nlm.nih.gov