Product Name

Phosphodiesterase, 3′,5′-cyclic-nucleotide-specific from bovine brain

General deion

Phosphodiesterase (PDE1) exists in two isoforms -PDE1A2 and PDE1B1. The enzyme has a role in the complex interaction between cyclic nucleotides and Ca₂₊ second-messenger systems.

Synonym

3′,5′-Cyclic-nucleotide 5′-nucleotidohydrolase from bovine brain, PDE

Assay

15-30 units/mg protein (in the presence of 0.03 mM Ca₂₊ and a saturating level (10 units per ml) of calmodulin); 1-5 units/mg protein (without added calmodulin)

CAS Number

9040-59-9

MDL number

MFCD00131864

Molecular Weight

mol wt ~60 kDa

Suitability

BioReagent

l Physical and Chemical Information

Appearance

lyophilized powder

l Biological Information

Biochem/Physiol Actions

Hydrolyzes the 3′,5′-phosphodiester in cyclic nucleotide monophosphates, such as cAMP and cGMP, to the corresponding nucleotide 5′-monophosphate.

Application

Phosphodiesterase, 3′,5′-cyclic-nucleotide-specific from bovine brain has been used to assay the protein activator, calmodulin and in in vitro adenylate cyclase assay.

Preparation Note

Affinity purified preparation which has been depleted of calmodulin.

Unit Definition

One unit will hydrolyze 1.0 μmole of 3′,5′-cyclic-AMP to 5′-AMP per min at pH 7.5 at 30 °C.

l Storage

Storage temp.

-20°C

l Precautions and Disclaimer

This product is for R&D use only, not for drug, household, or other uses.

l References

1. http://www.drugbank.ca

2. https://ncit.nci.nih.gov

3. https://www.ncbi.nlm.nih.gov