Product Name

Phosphodiesterase 3′,5′-Cyclic Nucleotide Activator-deficient from bovine heart

Synonym

3′,5′-Cyclic-nucleotide 5′-nucleotidohydrolase from bovine heart, PDE

Assay

Protein: ~75%; >0.1 units/mg protein (with added activator)

Molecular Weight

mol wt ~60 kDa

MDL number

MFCD00131864

Suitability

BioReagent

l Physical and Chemical Information

Physical form

lyophilized powder (contains imidazole buffer salts and magnesium sulfate)

l Biological Information

Biochem/Physiol Actions

Hydrolyzes the 3′,5′-phosphodiester bond in cyclic nucleotide monophosphates, such as cAMP and cGMP, to the corresponding nucleotide 5′-monophosphate.

The herbicide Atrazine was shown inhibit phosphodiesterase 3′,5′-Cyclic Nucleotide Activator-deficient from bovine heart.

Application

Cyclic nucleotide phosphodiesterase has been investigated for its mechanism of activation as a function of calmodulin and Ca_2+.It has also been used in a study to show that the binding of trifluoperazine to the activator of cyclic nucleotide phosphodiesterase increases the activity by over 10-fold.

For use in calmodulin assay. Activity is reduced to <50% without calmodulin present.

Preparation Note

Crude preparation which has been stripped of the protein activator, calmodulin.

Unit Definition

One unit will hydrolyze 1.0 μmole of 3′:5′,-cyclic-AMP to 5′-AMP per min at pH 7.5 at 30 °C.

l Storage

Storage temp.

-20°C

l Precautions and Disclaimer

This product is for R&D use only, not for drug, household, or other uses.

l References

1. http://www.drugbank.ca

2. https://ncit.nci.nih.gov

3. https://www.ncbi.nlm.nih.gov