Product Name

Sphingomyelinase from Bacillus cereus

Synonym

Sphingomyelin choline phosphohydrolase, Sphingomyelin phosphodiesterase

Assay

≥100 units/mg protein (Lowry)

CAS Number

9031-54-3

MDL number

MFCD00132362

Suitability

BioReagent

l Physical and Chemical Information

Appearance

Colorless to Light Yellow buffered aqueous glycerol solution

Physical form

Solution in 50% glycerol containing 50 mM Tris-HCl, pH 7.5.

l Biological Information

Biochem/Physiol Actions

Initiates the formation of sphingomyelin-based second messengers. Activates MAPK (mitogen-activated protein kinase) and SAPKs (stress-activated protein kinases); generates ceramide from sphingomyelin.

Application

Sphingomyelinase has been used in a study to assess the interaction of actin with the HIV-1 accessory protein Nef. Sphingomyelinase has also been used in a study to investigate X-ray scattering as a quality-control tool for liposomal drug-delivery systems.

Unit Definition

One unit will hydrolyze 1.0 μmole of TNPAL-sphingomyelin per min at pH 7.4 at 37 °C.

l Storage

Storage temp.

2-8°C

l Precautions and Disclaimer

This product is for R&D use only, not for drug, household, or other uses.

l References

1. http://www.drugbank.ca

2. https://ncit.nci.nih.gov

3. https://www.ncbi.nlm.nih.gov