Product Name

Sphingomyelinase from Bacillus cereus

General deion

Sphingomyelinase from Bacillus cereus (Bc-Smase) is a Mg₂₊-containing metalloenzyme. Bacillus cereus sphingomyelinase induces hemolysis of sheep erythrocytes.

Synonym

Sphingomyelin choline phosphohydrolase, Sphingomyelin phosphodiesterase

Assay

Protein:~10% Lowry; ≥100 units/mg protein

CAS Number

9031-54-3

MDL number

MFCD00132362

Suitability

BioReagent

l Physical and Chemical Information

Appearance

Faint Yellow to Dark Yellow to Dark Brown lyophilized powder

l Biological Information

Biochem/Physiol Actions

Initiates the formation of sphingomyelin-based second messengers. Activates MAPK (mitogen-activated protein kinase) and SAPKs (stress-activated protein kinases); generates ceramide from sphingomyelin.

Application

Sphingomyelinase has been used in a study to investigate skeletal manifestations in pediatric and adult patients with Niemann Pick disease.

Unit Definition

One unit will hydrolyze 1.0 μmole of TNPAL-sphingomyelin per min at pH 7.4 at 37 °C.

l Storage

Storage temp.

2-8°C

l Precautions and Disclaimer

This product is for R&D use only, not for drug, household, or other uses.

l References

1. http://www.drugbank.ca

2. https://ncit.nci.nih.gov

3. https://www.ncbi.nlm.nih.gov