Product Name

Phosphodiesterase I from Crotalus atrox (Western Diamondback Rattlesnake)

Synonym

5′-Exonuclease, Oligonucleate 5′-nucleotidohydrolase

Assay

Type IV, crude dried venom, ≥0.01 unit/mg solid

MDL number

MFCD00131883

Suitability

BioReagent

l Physical and Chemical Information

Appearance

crude dried venom

l Biological Information

Biochem/Physiol Actions

Phosphodiesterase I breaks phosphodiester bonds and catalyzes the hydrolysis of various nucleotide polyphosphates. Phosphodiesterase I is released from eucaryotic plasma membranes by phosphatidylinositol-specific phospholipase C.

Application

Phosphodiesterase (PDE) is any enzyme that is used to breaks phosphodiester bonds. Phosphodiesterase I, product P4506 from western diamondback rattlesnake, is used in phosphodiesterase activation assays for calmodulin. It is added to hydrolyze AMP. It is a membrane-bound glycoprotein that is used to catalyze the hydrolysis of various nucleotide polyphosphates.

Unit Definition

One unit will hydrolyze 1.0 μmole of bis(p-nitrophenyl) phosphate per min at pH 8.8 at 37 °C.

l Storage

Storage temp.

-20°C

l Precautions and Disclaimer

This product is for R&D use only, not for drug, household, or other uses.

l References

1. http://www.drugbank.ca

2. https://ncit.nci.nih.gov

3. https://www.ncbi.nlm.nih.gov