Product Name

Phosphodiesterase I from Crotalus adamanteus venom

Synonym

5′-Exonuclease, Oligonucleate 5′-nucleotidohydrolase

Assay

vial of ≥0.40 units, Purified

CAS Number

9025-82-5

MDL number

MFCD00131883

Suitability

BioReagent

l Physical and Chemical Information

Appearance

White to Off- White Powder

l Biological Information

Biochem/Physiol Actions

Phosphodiesterase I breaks phosphodiester bonds and catalyzes the hydrolysis of various nucleotide polyphosphates. Phosphodiesterase I is released from eucaryotic plasma membranes by phosphatidylinositol-specific phospholipase C.

Application

Phosphodiesterase (PDE) is any enzyme that is used to breaks phosphodiester bonds. It is a membrane-bound glycoprotein that is used to catalyze the hydrolysis of various nucleotide polyphosphates. Phosphodiesterase I is used in phosphodiesterase activation assays to hydrolyze AMP. Product P³²⁴³ is from Crotalus adamanteus venom and is purified. Product P³²⁴³ has been used to hydrolyze tRNA with wyosine derivatives into mononucleosides.

Preparation Note

Purified via the method of Williams, et al. and further treated to inactivate contaminating 5′-nucleotidase activity.

Unit Definition

One unit will hydrolyze 1.0 μmole of bis(p-nitrophenyl) phosphate per min at pH 8.8 at 37 °C.

l Storage

Storage temp.

-20°C

l Precautions and Disclaimer

This product is for R&D use only, not for drug, household, or other uses.

l References

1. http://www.drugbank.ca

2. https://ncit.nci.nih.gov

3. https://www.ncbi.nlm.nih.gov