Product Name

Nuclease micrococcal from Staphylococcus aureus

Synonym

Endonuclease micrococcal, MNase, Micrococcal endonuclease, Micrococcal nuclease

Assay

100-300 units/mg protein; Protein, ≥40% E1%/280 (Balance primarily sodium citrate)

CAS Number

9013-53-0

MDL number

MFCD00131731

Suitability

BioReagent

l Physical and Chemical Information

Appearance

White to White w/ Yellow Cast Lyophilized Powder

Solubility(25°C)

Clear to Slightly Hazy;Colorless to Faint Yellow; 0.90 - 1.10 mg/ml

l Biological Information

Biochem/Physiol Actions

Cleavage of DNA by Nuclease micrococcal is greater at the 5′ sides of A or T nucleotides than at G or C.

Hydrolyzes 5′-phosphodiester bonds of DNA.

Application

Nuclease from Staphylococcus aureus has been used in a study to assess coagulase and heat-resistant strains found in animals. It has also been used in a study to investigate the  characteristic of two genes in S. aureus that encode two thermostable nucleases.

Preparation Note

Reconstitute to a concentration of 1 unit per 5 µl with water.

Unit Definition

One unit will produce 1.0 μmole of acid soluble polynucleotides from native DNA per min at pH 8.8 at 37 °C, based on EM/260 = 10,000 for the mixed nucleotides.

l Storage

Storage temp.

-20°C

l Precautions and Disclaimer

This product is for R&D use only, not for drug, household, or other uses.

l References

1. http://www.drugbank.ca

2. https://ncit.nci.nih.gov

3. https://www.ncbi.nlm.nih.gov