Product Name

Nuclease(Endonuclease from Serratia marcescens)

Assay

≥250 units/μL, ≥90% (SDS-PAGE), recombinant, expressed in E. coli

CAS Number

9025-65-4

MDL number

MFCD00131010

Suitability

BioReagent

l Physical and Chemical Information

Appearance

Clear Colorless buffered aqueous glycerol solution

Physical form

Solution in 50% glycerol containing 20 mM Tris HCl, pH 8.0, 2 mM MgCl², and 20 mM NaCl.

l Biological Information

Biochem/Physiol Actions

Digests native or heat-denatured DNA and RNA.

Endonuclease from Serratia marcescens is a dimer of 30 kDa subunits with two essential disulfide bonds. This endonuclease attacks and degrades all forms of DNA and RNA (single stranded, double stranded, linear and circular) and is effective over a wide range of operating conditions. The optimum pH for enzyme activity is found to be 8.0-9.2. It completely digests nucleic acids to 5′- monophosphate terminated oligonucleotides 3 to 5 bases in length. This is ideal for removal of nucleic acids from recombinant proteins and for applications where complete digestion of nucleic acids is desirable. It also reduces viscosity in protein extracts and prevents cell clumping. Pre-treatment of a protein sample improves its resolution on 2D gel electrophoresis by eliminating any bound nucleic acids.

Application

Used for the removal of nucleic acid from protein samples.

Endonuclease from Serratia marcescens, can be used to degrade all forms of DNA and RNA while having no proteolytic activity. Benzonase nuclease can also be used to prepare proteins in microcalorimetric experiments.

The enzyme from Tomums has been used to limit cell clumping during the preparation of chimeric cell mixtures.It has also been used for the preparation of nuclear extracts by digesting DNA and releasing nuclear proteins intimately associated with DNA.

Unit Definition

One unit will digest sonicated salmon sperm DNA to acid-soluble oligonucleotides equivalent to a ΔA²⁶⁰ of 1.0 in 30 min at pH 8.0 at 37 °C (reaction volume 2.625 ml).

l Storage

Storage temp.

-20°C

l Precautions and Disclaimer

This product is for R&D use only, not for drug, household, or other uses.

l References

1. http://www.drugbank.ca

2. https://ncit.nci.nih.gov

3. https://www.ncbi.nlm.nih.gov