Product Name

Nuclease S1 from Aspergillus oryzae

Synonym

Endonuclease S1

Assay

≥100,000 units/ml, for single-strand DNA/RNA digestion

CAS Number

37288-25-8

MDL number

MFCD00131010

Suitability

BioReagent

l Physical and Chemical Information

Physical form

Solution containing 30 mM sodium acetate, 50 mM NaCl, 1 mM ZnCl², 50% glycerol, 2 mg/ml protein

l Biological Information

Biochem/Physiol Actions

Nuclease S1 isolated from Aspergillus oryzae exhibits endo- and exolytic hydrolytic activity for the phosphodiester bonds of single-stranded DNA and RNA yielding 5’-phosphomononucleotide and 5’-phosphooligonucleotide end-products. It is used to digest non-annealed polynucleotide tails and hairpin loops in RNA and DNA duplexes and can be used to convert superhelical DNA to the linear form.

SI nuclease from Aspergillus oryzae can generate double-stranded DNA breaks in response to DNA nicks or abasic sites.

Application

Nuclease S1 from Aspergillus oryzae has been used in a study to assess a biochemical method for mapping mutational alterations in DNA. It has also been used in a study to investigate the DNA damage and repair in a γ-irradiated rat brain tumor.

Unit Definition

One unit will cause 1.0 microgram of single-stranded nucleic acid to become perchloric acid soluble per minute at pH 4.6 at 37°C.

l Storage

Storage temp.

-20°C

l Precautions and Disclaimer

This product is for R&D use only, not for drug, household, or other uses.

l References

1. http://www.drugbank.ca

2. https://ncit.nci.nih.gov

3. https://www.ncbi.nlm.nih.gov