Product Name

5′-Nucleotidase human

General deion

5′-Nucleotidase is an extracellular enzyme found in a wide variety of mammalian cells. It helps to accelerate the hydrolysis reaction of phosphate group from 5′ nucleotides, resulting in corresponding nucleosides.

Synonym

CD73, NT5E, ecto-5′-nucleotidase

Assay

recombinant, expressed in CHO cells, vial of 6-12 μg; ≥90% (SDS-PAGE)

CAS Number

9027-73-0

Molecular Weight

mol wt ~61 kDa by SDS-PAGE (reducing)

MDL number

MFCD00130311

Suitability

BioReagent

l Physical and Chemical Information

Appearance

glycerol solution

Physical form

Supplied as a solution containing Tris, NaCl, CaCl², and 20% glycerol.

l Biological Information

Biochem/Physiol Actions

5′-nucleotidase, also known as CD73, is an extracellular enzyme that converts nucleoside-5′−monophosphates to nucleosides with a substrate preference of AMP. Native 5′-nucleotidase is a GPI-anchored protein whose exporession is upregulated by hypoxia. 5′-nucleotidase has many functions in vivo including the generation of extracellular adenosine.

5′-Nucleotidase has various clinical significances. It is a key molecule in the regulation of cancer cells proliferation, migration and invasion in vitro tumor angiogenesis, and tumor immune escape in vivo. Due to this important role, the enzyme is a potential target for cancer research. It is also involved in salvage of extracellular nucleotides and plays a key role in the control of tissue homeostasis.

Unit Definition

One unit will hydrolyze 1.0 μmole of inorganic phosphorus from adenosine 5′-monophosphate per min at pH 9.0 at 37 °C.

l Storage

Storage temp.

−70°C

l Precautions and Disclaimer

This product is for R&D use only, not for drug, household, or other uses.

l References

1. http://www.drugbank.ca

2. https://ncit.nci.nih.gov

3. https://www.ncbi.nlm.nih.gov