Product Name

Phosphatase, Acid from potato

Synonym

(Orthophosphoric-monoester phosphohydrolase acid optimum), Acid Phosphatase from potato

Assay

≥0.5 unit/mg solid

CAS Number

9001-77-8

MDL number

MFCD00131847

Suitability

BioReagent

l Physical and Chemical Information

Appearance

lyophilized powder

l Biological Information

Biochem/Physiol Actions

Acid phosphatase is a cytoplasmic lysosomal hydrolase that catalyzes the conversion of an orthophosphoric monoester and water to an alcohol and orthophosphate.

Application

Acid phosphatase is routinely used to dephosphorylate proteins and nucleic acids. Acid phosphatase is an acid hydrolase that normally resides in lysosomes. It is used as marker for the identification of lysosomes in subcellular fractionations. Acid phosphatase, has been used as a control in order to study how Mycobacterium bovis BCG effects the levels of acid phosphatase activity in alveolar macrophages.

Preparation Note

APase is soluble in cold water (0.15–0.25 unit/ml). Prepare solution immediately before use.

Unit Definition

One unit will hydrolyze 1.0 μmole of p-nitrophenyl phosphate per min at pH 4.8 at 37 °C.

l Storage

Storage temp.

-20°C

l Precautions and Disclaimer

This product is for R&D use only, not for drug, household, or other uses.

l References

1. http://www.drugbank.ca

2. https://ncit.nci.nih.gov

3. https://www.ncbi.nlm.nih.gov