Product Name

Phosphatase, Acid from wheat germ

Synonym

(Orthophosphoric-monoester phosphohydrolase [acid optimum] ), Acid Phosphatase from wheat germ

Assay

≥0.4 unit/mg solid; Contains lipase

CAS Number

9001-77-8

MDL number

MFCD00131847

Suitability

BioReagent

l Physical and Chemical Information

Appearance

solid

l Biological Information

Biochem/Physiol Actions

Plant acid phosphatase (APase) is localized in cytosol, vacuoles, and cell walls. Plant acid phosphatase is important for phosphate acquisition to mobilize organic phosphates in the soil. Phosphate starvation induces acid phosphatase generation. Acid phosphatase originates from the aleurone and scutellar tissues during germination. It hydrolyzes phytins, ATP, protein phosphates and nucleotide phosphates. It has a role in general metabolic reactions. Wheat embryo APase consists of four isoenzymes. The molecular mass of wheat germ APase is 58 kDa (gel filtration), the pH Optimum is 5.7, the pH Range is 4.0–7.0 and the optimal temperature is 45 °C.

Application

Acid phosphatase (APase) non-specifically catalyzes the hydrolysis of monoesters and anhydrides of phosphoric acid to produce inorganic phosphate. It is used to study the production, transport, and recycling of phosphate and the metabolic and energy transduction processes of the cell. Product P3627 is from wheat germ and has been used to determine the effect of phosphatase treatment on 3F3/2 staining.

Preparation Note

Prepared essentially by method of Singer, T.P., J. Biol. Chem., 174, 11 (1948).

Unit Definition

One unit will hydrolyze 1.0 μmole of p-nitrophenyl phosphate per min at pH 4.8 at 37 °C.

l Storage

Storage temp.

-20°C

l Precautions and Disclaimer

This product is for R&D use only, not for drug, household, or other uses.

l References

1. http://www.drugbank.ca

2. https://ncit.nci.nih.gov

3. https://www.ncbi.nlm.nih.gov