Product Name

Phosphatase, Alkaline from calf intestine

General deion

Alkaline phosphatase is a phosphomonoesterase that catalyzes the cleavage of terminal phosphates from nucleic acids. The free 5′-OH can be phosphorylated with polynucleotide kinase and γ32P-ATP to produce γ32P-end-labeled nucleic acids. Linearized cloning vectors can be prevented from recircularizing by dephosphorylation with alkaline phosphatase.

Synonym

CIAP, CIP, Orthophosphoric-monoester phosphohydrolase (alkaline optimum)

Assay

≥10,000 units/mL

CAS Number

9001-78-9

Molecular Weight

mol wt ~80 kDa

MDL number

MFCD00131849

Suitability

BioReagent

l Physical and Chemical Information

Appearance

buffered aqueous glycerol solution

l Biological Information

Application

Commonly used to remove the 5′-terminal phosphate from nucleic acids during molecular cloning reactions.

Unit Definition

One unit will hydrolyze 1 μmole of p-nitrophenyl phosphate per min at 37 °C.

l Storage

Storage temp.

-20°C

l Precautions and Disclaimer

This product is for R&D use only, not for drug, household, or other uses.

l References

1. http://www.drugbank.ca

2. https://ncit.nci.nih.gov

3. https://www.ncbi.nlm.nih.gov