Product Name

Phosphatase, Alkaline from porcine kidney

Synonym

Orthophosphoric-monoester phosphohydrolase (alkaline optimum)

Assay

≥100 DEA units/mg protein

CAS Number

9001-78-9

MDL number

MFCD00131849

Suitability

BioReagent

l Physical and Chemical Information

Appearance

White to Dark Yellow to Dark Brown Lyophilized Powder

Physical form

Lyophilized fromTris-HCl buffer, with magnesium chloride and zinc chloride, pH 8.0

l Biological Information

Biochem/Physiol Actions

Alkaline phosphatase is a hydrolase enzyme that dephosphorylates molecules such as nucleotides, proteins and alkaloids.

Application

Alkaline phosphatase is used for conjugation to antibodies and other proteins for ELISA, Western blotting, and histochemical detection. Alkaline phosphatase is also used to prevent DNA self ligation and for radiolabeling.

Unit Definition

One unit will hydrolyze 1.0 μmole of p-nitrophenyl phosphate per min at pH 9.8 at 37 °C.

l Storage

Storage temp.

-20°C

l Precautions and Disclaimer

This product is for R&D use only, not for drug, household, or other uses.

l References

1. http://www.drugbank.ca

2. https://ncit.nci.nih.gov

3. https://www.ncbi.nlm.nih.gov