Product Name

Phosphatase, Alkaline from bovine intestinal mucosa

Synonym

Alkaline phosphatase, Orthophosphoric-monoester phosphohydrolase (alkaline optimum)

Assay

≥5,500 DEA units/mg protein

CAS Number

9001-78-9

Molecular Weight

dimer mol wt ~160 kDa

MDL number

MFCD00131849

Suitability

BioReagent

l Physical and Chemical Information

Appearance

buffered aqueous glycerol solution

Physical form

Solution in 50% glycerol containing 5 mM Tris, 5 mM MgCl² and 0.1 mM ZnCl², pH 7.0

l Biological Information

Biochem/Physiol Actions

The enzyme has a broad specificity for phosphate esters of alcohols, amines, pyrophosphate, and phenols. It is routinely used to dephosphorylate proteins and nucleic acids.

Application

Alkaline phosphatase can be used to dephosphorylate casein and other proteins. Alkaline phosphatase may be also be used to dephosphorylate the 5′-termini of DNA or RNA to prevent self-ligation. DNA or RNA can also be tagged with radiolabeled phosphate (via T4 polynucleotide kinase) after dephosphorylation with alkaline phosphatase..

High specific activity grade recommended for antibody and protein conjugation.

Preparation Note

Dilute solutions of alkaline phosphatase should be prepared in 10 mM Trizma-HCl, pH 8.0, 1-5 mM MgCl², and 0.1-0.2 mM ZnCl². 50% Glycerol can be included for long-term storage at 2–8 °C.

Unit Definition

One DEA unit will hydrolyze 1 μmole of 4-nitrophenyl phosphate per minute at pH 9.8 at 37 °C. (One glycine unit is equivalent to ~3 DEA units)

l Storage

Storage temp.

2-8°C

l Precautions and Disclaimer

This product is for R&D use only, not for drug, household, or other uses.

l References

1. http://www.drugbank.ca

2. https://ncit.nci.nih.gov

3. https://www.ncbi.nlm.nih.gov