Product Name

Phosphatase, Alkaline shrimp

General deion

Shrimp alkaline phosphatase is a heat labile, hydrolase enzyme. It is a high specific activity alkaline phosphatase purified from a recombinant source. There are two preferred sites for enzyme activity: 5′ protruding, recessive and blunt 5′-termini. The enzyme is irreversibly heat inactivated at 65°C for 15 minutes.

Synonym

Orthophosphoric-monoester phosphohydrolase (alkaline optimum), Phosphatase, Alkaline from shrimp

Assay

≥1,000 DEA units/mL, recombinant, expressed in proprietary host

CAS Number

9001-78-9

MDL number

MFCD00131849

Suitability

BioReagent

l Physical and Chemical Information

Appearance

buffered aqueous glycerol solution

Physical form

solution in 50% glycerol containing 25 mM Tris-HCl, pH 7.5, 1 mM MgCl²

l Biological Information

Biochem/Physiol Actions

Alkaline phosphatase is commonly responsible for dephosphorylation of nucleotides, proteins and alkaloids. The enzyme nonspecifically catalyzes the dephosphorylation of 5′-termini DNA and RNA phosphomonoesters reactions but it does not affect diphosphate or triphosphate ages.

Application

Alkaline phosphatase was used to examine its role in the prevention of high-fat-diet-induced metabolic syndrome in mice.It also may be used in pasteurization process for milks used in dairy products.

Unit Definition

One unit will hydrolyze 1 μmole of 4-nitrophenyl phosphate per minute at pH 9.8 at 37 °C.

l Storage

Storage temp.

-20°C

l Precautions and Disclaimer

This product is for R&D use only, not for drug, household, or other uses.

l References

1. http://www.drugbank.ca

2. https://ncit.nci.nih.gov

3. https://www.ncbi.nlm.nih.gov