Product Name

Ribonuclease B from bovine pancreas

Synonym

RNase B

Assay

Protein: ≥80% (SDS-PAGE); ≥50 Kunitz units/mg protein

CAS Number

9001-99-4

MDL number

MFCD00132184

Suitability

BioReagent

l Physical and Chemical Information

Appearance

White to Light Yellow Powder

l Biological Information

Biochem/Physiol Actions

Native RNase BS generated by subtilisin digestion of native RNase B comprising of amino acid residues 21-124 of RNase B, is sensitive to PNGase F digestion. Intramolecular N-glycans of bovine pancreatic RNase B function like chaperone. RNase B is found to be much faster than RNase A, while RNase A is liable to aggregate during regeneration. The stimulatory effect of Asn-oligosaccharide (which corresponds to the most predominant sugar chain of RNase B) reveals that the N-glycans of RNase B facilitates the transformation of bulky intermediates into folded, compact species.

Application

Ribonuclease B from bovine pancreas is used in the digestion of RNA during cell cycle platform analysis.

Preparation Note

Purified by affinity chromatography

l Storage

Storage temp.

-20°C

l Precautions and Disclaimer

This product is for R&D use only, not for drug, household, or other uses.

l References

1. http://www.drugbank.ca

2. https://ncit.nci.nih.gov

3. https://www.ncbi.nlm.nih.gov