Product Name

Ribonuclease A from bovine pancreas

General deion

Ribonucleases do not hydrolyze DNA, because the DNA lacks 2′-OH groups essential for the formation of cyclic intermediates. RNase can hydrolyze RNA from protein samples. Pancreatic RNase A specifically cleaves at the 3′-side of pyrimidine (uracil or cytosine) phosphate bonds.

Synonym

Pancreatic Ribonuclease, RNase A, Ribonucleate 3′-pyrimidinooligonucleotidohydrolase

Assay

≥90% (SDS-PAGE), 75-125 Kunitz units/mg protein; Type XII-A

CAS Number

9001-99-4

Molecular Weight

mol wt ~13,700

MDL number

MFCD00132181

Suitability

BioReagent

l Physical and Chemical Information

Appearance

White to Off-White lyophilized powder

l Biological Information

Application

Ribonuclease A is used to remove RNA from DNA plasmid preparations and protein samples. It is also used in RNA sequence analysis and protection assays. Ribonuclease A is used to remove RNA from DNA plasmid preparations and protein samples. Ribonuclease A is used for RNase protection assays, to remove unspecifically bound RNA, analysis of RNA sequences, to hydrolyze RNA contained in protein samples, and the purification of DNA. Ribonuclease A from bovine pancreas has been used in a study to determine that both PDI and PDIp can attack the native disulfide bonds in thermally-unfolded RNase and form stable disulfide-ed complexes. Ribonuclease A from bovine pancreas has also been used in a study to investigate the stepwise deamidation of ribonuclease A at five sites.

Preparation Note

A further chromatographic purification of R5503 to yield essentially pure RNAse A

l Storage

Storage temp.

-20°C

l Precautions and Disclaimer

This product is for R&D use only, not for drug, household, or other uses.

l References

1. http://www.drugbank.ca

2. https://ncit.nci.nih.gov

3. https://www.ncbi.nlm.nih.gov