Product Name

Ribonuclease A from bovine pancreas

General deion

RNase A is an endoribonuclease that attacks at the 3’OHphosphate of a pyrimidine nucleotide. The sequence of pG-pG-pC-pA-pG will be cleaved to give pG-pG-pCp and A-pG. The highest activity is exhibited with single stranded RNA.

Synonym

Pancreatic Ribonuclease, RNase A, Ribonucleate 3′-pyrimidinooligonucleotidohydrolase

Assay

Protein: 20-40 mg/mL;

≥Kunitz units/mg protein

CAS Number

9001-99-4

Molecular Weight

mol wt 13.7 kDa; mol wt ~13,700

MDL number

MFCD00132181

Suitability

BioReagent, for molecular biology

l Physical and Chemical Information

Appearance

Clear to Slightly Hazy Colorless to Light Tan Liquid

Physical form

Solution of 50% glycerol, 10mM Tris-HCL pH 8.0

l Biological Information

Application

Suitable for:

• RNase protection assays

• Removal of unspecifically bound RNA

• Analysis of RNA sequences

• Hydrolysis of RNA contained in protein samples

• Plasmid DNA purification

Ribonuclease A is used to remove RNA from DNA plasmid preparations and protein samples. It is also used in RNA sequence analysis and protection assays.

Unit Definition

A major application for RNase A is the removal of RNA from preparations of plasmid DNA. For this application, DNase free RNase A is used at a final concentration of 10 ug/mL. Boiling stock solutions of this RNase A product to inactivate residual DNase is not necessary and may cause precipitation of RNase and possible loss of enzymatic activity. If an RNase A solution is heated at a neutral pH, precipitation will occur. When heated at a lower pH, some precipitation may occur because of protein impurities that are present.

l Storage

Storage temp.

-20°C

l Precautions and Disclaimer

This product is for R&D use only, not for drug, household, or other uses.

l References

1. http://www.drugbank.ca

2. https://ncit.nci.nih.gov

3. https://www.ncbi.nlm.nih.gov