Product Name

Ribonuclease T1 from Aspergillus oryzae

Synonym

Guanyloribonuclease, Ribonucleate 3′-guanylo-oligonucleotidohydrolase

Assay

300,000-600,000 units/mg protein

CAS Number

9026-12-4

MDL number

MFCD00132191

Suitability

BioReagent

l Physical and Chemical Information

Appearance

Colorless to Light Yellow to Light Beige ammonium sulfate suspension

Physical form

Suspension in 2.8 M (NH⁴)²SO⁴ solution

l Biological Information

Biochem/Physiol Actions

Ribonuclease T1 (RNase T1) from Aspergillus oryzae is an endoribonuclease that hydrolyzes after G residues. Cleavage occurs between the 3’-phosphate group of a guanidine ribonucleotide and 5’-hydroxyl of the adjacent nucleotide. The initial product is a 2’:3’ cyclic phosphate nucleoside that is hydrolyzed to the corresponding 3’-nucleoside phosphate. It differs from Pancreatic RNase in that it attacks the guanine sites specifically to yield 3′-GMP and oligonucleotides with a 3′-GMP terminal group.

Application

Ribonuclease T1 (RNase T1) from Aspergillus oryzae is used to digest denatured RNA prior to sequencing and is used for protein folding studies.

Unit Definition

One unit will produce acid soluble oligonucleotides equivalent to a ΔA²⁶⁰ of 1.0 in 15 min at pH 7.5 at 37°C, in a reaction volume of 1.0 mL. Substrate: Yeast RNA.

l Storage

Storage temp.

2-8°C

l Precautions and Disclaimer

This product is for R&D use only, not for drug, household, or other uses.

l References

1. http://www.drugbank.ca

2. https://ncit.nci.nih.gov

3. https://www.ncbi.nlm.nih.gov