Product Name

Deoxyribonuclease II from bovine spleen

Synonym

DNase II, Deoxyribonucleate 3′-oligonucleotido-hydrolase

Assay

≥1,000 units/mg protein; Type V, essentially salt-free

CAS Number

9025-64-3

MDL number

MFCD00130919

Suitability

BioReagent

l Physical and Chemical Information

Appearance

White to Light Brown lyophilized powder

l Biological Information

Biochem/Physiol Actions

Deoxyribonuclease II, also called as acid DNAse, hydrolyzes deoxyribonucleotide ages in native and denatured DNA yielding products with 3′-phosphates. In vitro, its optimum pH range is 4.5 - 5.0. It also acts upon p-nitrophenyl-phosphodiesters at pH 5.6 - 5.9. The molecular weight is approximately 38,000 Da.

Application

DNAse II from Sigma has been used in the dissociation medium during the preparation of embryonic cardiac myocytes from rat heart. Deoxyribonuclease II from bovine spleen has been used in a study that conducted a partial purification of deoxyribonucleases from eggs and liver of Xenopus laevis. Deoxyribonuclease II from bovine spleen has also been used in a study to investigate nucleic acid and protein synthesis of splenic lymphocytes.

Unit Definition

One Kunitz unit will produce a ΔA²⁶⁰ of 0.001 per min per mL at pH 4.6 at 25 °C; [Mg₂₊] = 0.83 mM

l Storage

Storage temp.

-20°C

l Precautions and Disclaimer

This product is for R&D use only, not for drug, household, or other uses.

l References

1. http://www.drugbank.ca

2. https://ncit.nci.nih.gov

3. https://www.ncbi.nlm.nih.gov