Product Name

Deoxyribonuclease II from porcine spleen

Synonym

DNase II, Deoxyribonucleate 3′-oligonucleotido-hydrolase

Assay

2,000-6,000 Kunitz units/mg protein (biuret); Type IV

CAS Number

9025-64-3

MDL number

MFCD00130919

Suitability

BioReagent

l Physical and Chemical Information

Appearance

lyophilized powder

Physical form

Contains sodium chloride

l Biological Information

Biochem/Physiol Actions

The enzyme is a lysosomal acid hydrolase that hydrolyzes DNA to 3′-phosphoryl oligonucleotides in the absence of metal ions, under acidic conditions. It is a non-covalently ed α·β heterodimer of approximately 45,000 Da molecular mass.

Application

DNase II from Sigma has been used to treat transformed cells during the purification of β-lactamase. It has also been used for the preparation of adenoma tissue in a study that investigated the effect of somatoprim on growth hormone secretion in human adenoma cell cultures (hSA).

Deoxyribonuclease II from porcine spleen has been used in an immunohistological study of the immune system cells in paraffin-embedded tissues. Deoxyribonuclease II from porcine spleen has also been used in a study to investigate its reassociation with the lysosomal membrane.

Unit Definition

One Kunitz unit will produce a ΔA²⁶⁰ of 0.001 per min per mL at pH 4.6 at 25°C; [Mg₂₊] = 0.83 mM

l Storage

Storage temp.

-20°C

l Precautions and Disclaimer

This product is for R&D use only, not for drug, household, or other uses.

l References

1. http://www.drugbank.ca

2. https://ncit.nci.nih.gov

3. https://www.ncbi.nlm.nih.gov