Product Name

Deoxyribonuclease I bovine

Synonym

DNAse I, Deoxyribonucleate 5′-oligonucleotido-hydrolase

Assay

≥5,000 units/mg protein; recombinant, expressed in Pichia pastoris

Molecular Weight

mol wt ~39 kDa

MDL number

MFCD00130918

Suitability

BioReagent

l Physical and Chemical Information

Appearance

Clear buffered aqueous glycerol solution

Physical form

Supplied as a solution in 4 mg/ml glycine pH 5.0, 5 mM calcium acetate and 50% glycerol

l Biological Information

Biochem/Physiol Actions

Digests single- and double-stranded DNA to a mixture of mono- and oligonucleotides carrying 5′ phosphates and 3′ OH termini. This catalytic activity is divalent ion-dependent. In the presence of Mg₂₊, DNase I hydrolyzes each strand of double-stranded DNA randomly and independently. In the presence of Mn₂₊, both strands can be cleaved.

DNase I is an endonuclease that acts on phosphodiester bonds (adjacent to pyrimidines) to produce polynucleotides with terminal 5′-phosphates. The pH optimum is found to be between 7 and 8. Divalent cations such as Mn₂₊, Ca₂₊, Co₂₊, and Zn₂₊ are activators of the enzyme. A concentration of 5 mM Ca₂₊ stabilizes the enzyme against proteolytic digestion. 2-Mercaptoethanol, chelators, sodium dodecyl sulfate (SDS) and actin are known to inhibit the enzyme activity.

Application

Used for the removal of DNA from protein samples.

DNAse I from Sigma was used to treat nuclear lysate to obtain single nucleosomes in a study. The enzyme has also been for the preparation and harvest of mice mammary glands.

Deoxyribonuclease I bovine has been used in a study to compare the initial actions of spleen deoxyribonuclease and pancreatic deoxyribonuclease. Deoxyribonuclease I bovine has also been used in a study to investigate deoxythymidine 3′, 5′-di-p-nitrophenyl phosphate as a synthetic substrate for bovine pancreatic deoxyribonuclease.

Preparation Note

Produced without using any animal cells or animal derived materials.

Unit Definition

One unit will produce a ΔA²⁶⁰ of 0.001 per min per mL reaction mixture using calf thymus DNA at pH 5.0 and 25°C

l Storage

Storage temp.

-20°C

l Precautions and Disclaimer

This product is for R&D use only, not for drug, household, or other uses.

l References

1. http://www.drugbank.ca

2. https://ncit.nci.nih.gov

3. https://www.ncbi.nlm.nih.gov