Product Name

Acetylcholinesterase from Electrophorus electricus (electric eel)

General deion

Molecular Weight: 280 kDa

Isoelectric Point: 5.5

Extinction Coefficient: E_1% = 18.0 (280 nm)

Acetylcholinesterase from Electrophorus electricus is a tetramer composed of 4 equal subunits of 70 kDa each. Each subunit contains one active site. The enzyme is a glycoprotein containing hexosamines.

Synonym

AChE, Acetylcholine acetylhydrolase, Cholinesterase, Acetyl

Assay

200-1,000 units/mg protein; Type VI-S

CAS Number

9000-81-1

MDL number

MFCD00081268

Suitability

BioReagent

l Physical and Chemical Information

Appearance

lyophilized powder

Physical form

Lyophilized powder containing Tris buffer salts

Solubility(25°C)

20 mM Tris HCl buffer, pH 7.5: soluble 1.0 mg/mL, clear(lit.)

l Biological Information

Biochem/Physiol Actions

Major degradative enzyme for acetylcholine in vivo. Converts acetylcholine + H²O to choline + acetic acid.

Application

The enzyme from sigma has been used as a reference to to evaluate the effect of aspartame metabolites on hippocampal acetylcholinesterase activity. The enzyme has also been used in immobilization studies for the rapid detection of acetylthiocholine chloride.

Preparation Note

This enzyme dissolves in 20 mM Tris-HCl buffer, pH 7.5 at 1 mg/mL concentration, yielding a clear solution.

Unit Definition

One unit will hydrolyze 1.0 μmole of acetylcholine to choline and acetate per min at pH 8.0 at 37 °C.

l Storage

Storage temp.

-20°C

l Precautions and Disclaimer

This product is for R&D use only, not for drug, household, or other uses.

l References

1. http://www.drugbank.ca

2. https://ncit.nci.nih.gov

3. https://www.ncbi.nlm.nih.gov