Product Name

Acetylcholinesterase from Electrophorus electricus (electric eel)

General deion

Molecular Weight: 280 kDa

Isoelectric Point: 5.5

Extinction Coefficient: E_1% = 18.0 (280 nm)

Acetylcholinesterase from Electrophorus electricus is a tetramer composed of 4 equal subunits of 70 kDa each. Each subunit contains one active site. The enzyme is a glycoprotein containing hexosamines.

Synonym

AChE, Acetylcholine acetylhydrolase, Cholinesterase, Acetyl

Assay

≥1,000 units/mg protein; Type V-S

CAS Number

9000-81-1

MDL number

MFCD00081268

Suitability

BioReagent

l Physical and Chemical Information

Appearance

Faint Yellow to Yellow to Beige lyophilized powder

Physical form

Lyophilized powder containing Tris buffer salts

Solubility(25℃)

Tris buffer: 1 mg/mL (0.02 M Tris buffer, pH 7.5)

l Biological Information

Biochem/Physiol Actions

Major degradative enzyme for acetylcholine in vivo. Converts acetylcholine + H²O to choline + acetic acid.

Preparation Note

The enzyme is soluble in water (1 mg/mL), and is also soluble in 0.1 M Trizma-HCl, pH 7.5 (2 mg/mL), yielding a clear solution. The enzyme can be solubilized and diluted in 0.02 M sodium phosphate buffer, pH 7.0. For dilute enzyme solutions (1 mg/mL), add 1 mg/mL of BSA to stabilize the enzyme.

Caution

For dilute solutions (<1 mg/ml), add 1 mg/ml BSA to stabilize. Solutions containing at least 1 mg protein/ml in dilute neutral phosphate buffer are stable at least 6 months refrigerated.

Unit Definition

One unit will hydrolyze 1.0 μmole of acetylcholine to choline and acetate per min at pH 8.0 at 37 °C.

l Storage

Storage temp.

-20°C

l Precautions and Disclaimer

This product is for R&D use only, not for drug, household, or other uses.

l References

1. http://www.drugbank.ca

2. https://ncit.nci.nih.gov

3. https://www.ncbi.nlm.nih.gov