Product Name

Acetylcholinesterase from human erythrocytes

General deion

Predominantly exists as a tetrameric glycoprotein composed of disulfide-ed homodimers with a monomer MW of ~80 kDa.

Synonym

AChE, Acetylcholine acetylhydrolase

Assay

≥500 units/mg protein (BCA)

CAS Number

9000-81-1

MDL number

MFCD00081268

Suitability

BioReagent

l Physical and Chemical Information

Appearance

Colorless buffered aqueous solution

l Biological Information

Biochem/Physiol Actions

Acetylcholinesterase is the major in vivo degradative enzyme for acetylcholine. It converts acetylcholine and water to choline and acetic acid. Cholinesterases are inhibited by the natural carbamate alkaloid, eserine or physostigmine.

In blood there are two cholinesterases present: The erythrocyte associated enzyme, which is a true cholinesterase or acetylcholinesterase [(AChE) - E.C. 3.1.1.7], the serum associated enzyme, which is Pseudocholinesterase or Butyrylcholinesterase [(BuChE) - EC 3.1.1.8].

AChE is an ectoenzyme, anchored to the erythrocyte membrane via a GPI moiety.

Application

Acetylcholinesterase (AChE) from Sigma has been used in the structure-activity study of phosphoramido acid esters as inhibitors of AChE.

Preparation Note

The enzyme is the amphiphilic form extracted together with its GPI anchor with the aid of TRITON X-100 and purified by affinity chromatography.

Unit Definition

One unit will hydrolyze 1.0 μmole of acetylthiocholine iodide per min at pH 7.4 at 37 °C.

l Storage

Storage temp.

2-8°C

l Precautions and Disclaimer

This product is for R&D use only, not for drug, household, or other uses.

l References

1. http://www.drugbank.ca

2. https://ncit.nci.nih.gov

3. https://www.ncbi.nlm.nih.gov