Product Name

Phospholipase A² from honey bee venom (Apis mellifera)

General deion

Phospholipase A² from honey bee venom (Apis mellifera) is a calcium-dependent lipolytic enzyme.

Synonym

Lecithinase A, PLA², Phosphatidylcholine 2-acylhydrolase

Assay

600-2400 units/mg protein; salt-free

CAS Number

9001-84-7

MDL number

MFCD00131890

Suitability

BioReagent

l Physical and Chemical Information

Appearance

White to grey lyophilized powder

l Biological Information

Biochem/Physiol Actions

Hydrolyzes the β-ester bond of zwitterionic glycerophospholipids. Preferred substrates are phosphatidylcholine, phosphatidylethanolamine, and their plasmalogen analogues. Phosphatidylinositol and phosphatidylserine are also hydrolyzed. Aggressively attacks phospholipids in membranes of intact cells.

Application

Phospholipase A² from honey bee venom (Apis mellifera) has been used

• to determine renal proximal tubular segments (PTS) viability during oxygenation and hypoxia-reoxygenation

• in the treatment of photosystem-II (PSII) dimer complex of Synechocystis to study changes in oxygen-evolving activity

• as a standard in phospholipase activity assay

Preparation Note

Sigma-Aldrich tests the solubility of this product at 1.0 mg/mL in water. One publication reports the preparation of stock solutions of this product at the equivalent of 17.5 mg/mL in an aqueous buffer that contains 10 mM MOPS, pH 7.2, 0.5 mM EGTA, 100 mM HCl, and 4 mM CaCl².

Unit Definition

One unit will hydrolyze 1.0 μmole of soybean L-α-phosphatidylcholine to L-α-lysophosphatidylcholine and a fatty acid per min at pH 8.9 at 25 °C.

l Storage

Storage temp.

-20°C

l Precautions and Disclaimer

This product is for R&D use only, not for drug, household, or other uses.

l References

1. http://www.drugbank.ca

2. https://ncit.nci.nih.gov

3. https://www.ncbi.nlm.nih.gov