Product Name

Phospholipase A² from porcine pancreas

General deion

Phospholipase A² is a small disulfide-rich protein having 124 residues. It is a calcium-dependent enzyme.

Synonym

Lecithinase A, PLA², Phosphatidylcholine 2-acylhydrolase

Assay

≥600 units/mg protein

CAS Number

9001-84-7

MDL number

MFCD00131890

Suitability

BioReagent

l Physical and Chemical Information

Appearance

White to Off-White ammonium sulfate suspension

Physical form

Suspension in 3.2 M (NH⁴)²SO⁴ solution, pH 5.5

l Biological Information

Biochem/Physiol Actions

Hydrolyzes the β-ester bond of zwitterionic glycerophospholipids. Preferred substrates are phosphatidylcholine, phosphatidylethanolamine, and their plasmalogen analogues. Phosphatidylinositol and phosphatidylserine are also hydrolyzed. Aggressively attacks phospholipids in membranes of intact cells. It has a high catalytic activity on aggregated substrates compared to monomeric substrates.

Application

Phospholipase A₂ has been used in phospholipase assay and to determine rat renal proximal tubular segments (PTS) viability during oxygenation and hypoxia-reoxygenation.

Preparation Note

This product is soluble in water (1 mg/ml), yielding a clear, colorless solution.

Unit Definition

One unit will hydrolyze 1.0 μmole of soybean L-α-phosphatidylcholine to L-α-lysophosphatidylcholine and a fatty acid per min at pH 8.0 at 37 °C.

l Storage

Storage temp.

2-8°C

l Precautions and Disclaimer

This product is for R&D use only, not for drug, household, or other uses.

l References

1. http://www.drugbank.ca

2. https://ncit.nci.nih.gov

3. https://www.ncbi.nlm.nih.gov